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Article

Overcoming Near-Cognate Suppression in a Release Factor 1-Deficient Host with an Improved Nitro-Tyrosine tRNA Synthetase.

Journal of molecular biology - 24 Jul 2020

Beyer Jenna N, Hosseinzadeh Parisa, Gottfried-Lee Ilana, Van Fossen Elise M, Zhu Phillip, Bednar Riley M, Karplus P Andrew, Mehl Ryan A, Cooley Richard B

Abstract excerpt

Genetic code expansion (GCE) technologies incorporate non-canonical amino acids (ncAAs) into proteins at amber stop codons. To avoid unwanted truncated protein and improve ncAA-protein yields, genomically recoded strains of Escherichia coli lacking Release Factor 1 (RF1) are becoming increasingly popular expression hosts for GCE applications. In the absence of RF1, however, endogenous near-cognate amber...

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Identifiers

PMID: 32569745
DOI: 10.1016/j.jmb.2020.06.014
PMCID: PMC7665880

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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