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Article

A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli.

Journal of biochemistry - 1 Mar 2013

Ikeda-Boku Akiyoshi, Ohno Satoshi, Hibino Yuuka, Yokogawa Takashi, Hayashi Nobuhiro, Nishikawa Kazuya

Abstract excerpt

We developed an efficient method for introduction of 3-azidotyrosine (N(3)-Y) into proteins in Escherichia coli cells. We constructed a plasmid that is adaptable for the constitutive expression of both Methanosarcina acetivorans tyrosyl-tRNA synthetase (TyrRS) and tRNA(()(CUA)), and made an ortho...

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PMID: 23316081
DOI: 10.1093/jb/mvs153

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23316081-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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