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Article

Primer extension mutagenesis powered by selective rolling circle amplification.

PloS one - 1 Jan 2012

Huovinen Tuomas, Brockmann Eeva-Christine, Akter Sultana, Perez-Gamarra Susan, Ylä-Pelto Jani, Liu Yuan, Lamminmäki Urpo

Abstract excerpt

Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosylase treatment and rolling circle amplification (RCA) steps. It is shown that removal of uracil bases from the...

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Identifiers

PMID: 22355397
DOI: 10.1371/journal.pone.0031817
PMCID: PMC3280210

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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