A new set of reagents and related software used for NGS based classical and non-classical HLA typing showing evidence for a greater HLA haplotype diversity.

doi:10.1016/j.humimm.2020.02.003

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Article

A new set of reagents and related software used for NGS based classical and non-classical HLA typing showing evidence for a greater HLA haplotype diversity.

Human immunology - 1 May 2020

Alizadeh M, Picard C, Frassati C, Walencik A, Gauthier A Cesbron, Bennasar F, Verite F, Semana G

Abstract excerpt

To evaluate the HLA typing performance of a new Long-Range PCR NGS set of reagents and its dedicated software, a panel of 41 reference homozygous cell lines from the International Histocompatibility Working Group (IHWG) and a panel of 376 volunteer bone marrow donors were analyzed for classical and non-classical HLA class I and class II genes. All results, except HLA-DPB1, were obtained without any ambiguities at...

Topics

Alleles
Bone Marrow
Gene Frequency
Genes, MHC Class I
Genes, MHC Class II
HLA Antigens
Haplotypes
High-Throughput Nucleotide Sequencing
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Histocompatibility Testing

Humans

Indicators and Reagents

Linkage Disequilibrium

Polymerase Chain Reaction

Polymorphism, Genetic

Software

Tissue Donors

Source

Open in PubMed

Identifiers

PMID: 32122686
DOI: 10.1016/j.humimm.2020.02.003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-32122686-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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