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Article

Dual-molecular barcode sequencing detects rare variants in tumor and cell free DNA in plasma.

Scientific reports - 25 Feb 2020

Hirotsu Yosuke, Otake Sotaro, Ohyama Hiroshi, Amemiya Kenji, Higuchi Rumi, Oyama Toshio, Mochizuki Hitoshi, Goto Taichiro, Omata Masao

Abstract excerpt

Conventional next generation sequencing analysis has provided important insights into cancer genetics. However, the detection of rare (low allele fraction) variants remains difficult because of the error-prone nucleotide changes derived from sequencing/PCR errors. To eliminate the false-positive variants and detect genuine rare variants, sequencing technology combined with molecular barcodes will be useful. Here,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32099048
DOI: 10.1038/s41598-020-60361-3
PMCID: PMC7042261

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