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Article

Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing.

Nucleic acids research - 20 Jun 2016

Ståhlberg Anders, Krzyzanowski Paul M, Jackson Jennifer B, Egyud Matthew, Stein Lincoln, Godfrey Tony E

Abstract excerpt

Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be extremely low in these samples and ultra-sensitive methods are required for their detection. Here, we report an extremely simple and fast method for introduction of barcodes into DNA libraries made from 5 ng of DNA. Barcoded adapter...

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Identifiers

PMID: 27060140
DOI: 10.1093/nar/gkw224
PMCID: PMC4914102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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