Article
High-fidelity target sequencing of individual molecules identified using barcode sequences: de novo detection and absolute quantitation of mutations in plasma cell-free DNA from cancer patients.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Aug 2015
Kukita Yoji, Matoba Ryo, Uchida Junji, Hamakawa Takuya, Doki Yuichiro, Imamura Fumio, Kato Kikuya
Abstract excerpt
Circulating tumour DNA (ctDNA) is an emerging field of cancer research. However, current ctDNA analysis is usually restricted to one or a few mutation sites due to technical limitations. In the case of massively parallel DNA sequencers, the number of false positives caused by a high read error rate is a major problem. In addition, the final sequence reads do not represent the original DNA population due to the...
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