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Article

Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution.

Applied microbiology and biotechnology - 1 Aug 2011

Liu Yu Huan, Ye Mao, Lu Yi, Zhang Xia, Li Gang

Abstract excerpt

To obtain better performing laccases for textile dyes decolorization, random mutagenesis of Lac591, a metagenome-derived alkaline laccase, was carried out. After three rounds of error-prone PCR and high-throughput screening by assaying enzymatic activity toward the phenolic substrate 2,6-dimethoxyphenol (2,6-DMP), a mutant (Lac3T93) with remarkably improved enzymatic activity was obtained. Sequence analysis...

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PMID: 21523474
DOI: 10.1007/s00253-011-3292-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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