Article
Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus.
Applied and environmental microbiology - 1 Mar 2012
Camarero S, Pardo I, Cañas A I, Molina P, Record E, Martínez A T, Martínez M J, Alcalde M
Abstract excerpt
While the Pycnoporus cinnabarinus laccase (PcL) is one of the most promising high-redox-potential enzymes for environmental biocatalysis, its practical use has to date remained limited due to the lack of directed evolution platforms with which to improve its features. Here, we describe the construction of a PcL fusion gene and the optimization of conditions to induce its functional expression in Saccharomyces...
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