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PAQMAN: Protein-nucleic acid affinity quantification by MAss spectrometry in nuclear extracts.

Methods (San Diego, Calif.) - 1 Dec 2020

Gräwe Cathrin, Makowski Matthew M, Vermeulen Michiel

Abstract excerpt

In recent years, various mass spectrometry-based approaches have been developed to determine global protein-DNA binding specificities using DNA affinity purifications from crude nuclear extracts. However, these assays are semi-quantitative and do not provide information about interaction affinities. We recently developed a technology that we call Protein-nucleic acid Affinity Quantification by MAss spectrometry...

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PMID: 31857188
DOI: 10.1016/j.ymeth.2019.12.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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