Article
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
Nature biotechnology - 1 Feb 2020
Chen Haiqi, Liu Sophia, Padula Samuel, Lesman Daniel, Griswold Kettner, Lin Allen, Zhao Tongtong, Marshall Jamie L, Chen Fei
Abstract excerpt
Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally...
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