Article
Mutation dynamics and fitness effects followed in single cells.
Science (New York, N.Y.) - 16 Mar 2018
Robert Lydia, Ollion Jean, Robert Jerome, Song Xiaohu, Matic Ivan, Elez Marina
Abstract excerpt
Mutations have been investigated for more than a century but remain difficult to observe directly in single cells, which limits the characterization of their dynamics and fitness effects. By combining microfluidics, time-lapse imaging, and a fluorescent tag of the mismatch repair system in Escherichia coli, we visualized the emergence of mutations in single cells, revealing Poissonian dynamics. Concomitantly, we...
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