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Article

High-throughput RNAi screening by time-lapse imaging of live human cells.

Nature methods - 1 May 2006

Neumann Beate, Held Michael, Liebel Urban, Erfle Holger, Rogers Phill, Pepperkok Rainer, Ellenberg Jan

Abstract excerpt

RNA interference (RNAi) is a powerful tool to study gene function in cultured cells. Transfected cell microarrays in principle allow high-throughput phenotypic analysis after gene knockdown by microscopy. But bottlenecks in imaging and data analysis have limited such high-content screens to endpoint assays in fixed cells and determination of global parameters such as viability. Here we have overcome these...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 16628209
DOI: 10.1038/nmeth876

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