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Article

Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes.

Nature communications - 16 Dec 2019

Thibault Derek, Jensen Paul A, Wood Stephen, Qabar Christine, Clark Stacie, Shainheit Mara G, Isberg Ralph R, van Opijnen Tim

Abstract excerpt

While Tn-Seq is a powerful tool to determine genome-wide bacterial fitness in high-throughput, culturing transposon-mutant libraries in pools can mask community or other complex single-cell phenotypes. Droplet Tn-Seq (dTn-Seq) solves this problem by microfluidics facilitated encapsulation of individual transposon mutants into growth medium-in-oil droplets, thereby enabling isolated growth, free from the influence...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 31844066
DOI: 10.1038/s41467-019-13719-9
PMCID: PMC6914776

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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