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Article

Development of "Plug and Play" Fiducial Marks for Structural Studies of GPCR Signaling Complexes by Single-Particle Cryo-EM.

Structure (London, England : 1993) - 3 Dec 2019

Dutka Przemyslaw, Mukherjee Somnath, Gao Xiang, Kang Yanyong, de Waal Parker W, Wang Lei, Zhuang Youwen, Melcher Karsten, Zhang Cheng, Xu H Eric, Kossiakoff Anthony A

Abstract excerpt

"Universal" synthetic antibody (sAB)-based fiducial marks have been generated by customized phage display selections to facilitate the rapid structure determination of G protein-coupled receptor (GPCR) signaling complexes by single-particle cryo-electron microscopy (SP cryo-EM). sABs were generated to the two major G protein subclasses: trimeric Gi and Gs, as well as mini-Gs, and were tested to ensure binding in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 31669042
DOI: 10.1016/j.str.2019.10.004
PMCID: PMC7297049

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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