Article
Phage display uncovers a sequence motif that drives polypeptide binding to a conserved regulatory exosite of O-GlcNAc transferase.
Proceedings of the National Academy of Sciences of the United States of America - 17 Oct 2023
Alteen Matthew G, Meek Richard W, Kolappan Subramania, Busmann Jil A, Cao Jessica, O'Gara Zoe, Chou Ying, Derda Ratmir, Davies Gideon J, Vocadlo David J
Abstract excerpt
The modification of nucleocytoplasmic proteins by O-linked N-acetylglucosamine (O-GlcNAc) is an important regulator of cell physiology. O-GlcNAc is installed on over a thousand proteins by just one enzyme, O-GlcNAc transferase (OGT). How OGT is regulated is therefore a topic of interest. To gain insight into these questions, we used OGT to perform phage display selection from an unbiased library of ~109 peptides...
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