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Article

Specialized transduction designed for precise high-throughput unmarked deletions in Mycobacterium tuberculosis.

mBio - 3 Jun 2014

Jain Paras, Hsu Tsungda, Arai Masayoshi, Biermann Karolin, Thaler David S, Nguyen Andrew, González Pablo A, Tufariello Joann M, Kriakov Jordan, Chen Bing, Larsen Michelle H, Jacobs William R

Abstract excerpt

UNLABELLED: Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a temperature-sensitive shuttle phasmid with a greater cloning capacity than phAE87, and (iii) bacteriophage-mediated...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 24895308
DOI: 10.1128/mBio.01245-14
PMCID: PMC4049104

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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