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Article

Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development.

Stem cell reports - 13 Nov 2018

Chen Zhenyu, Ren Xudong, Xu Xiangjie, Zhang Xiaojie, Hui Yi, Liu Zhongliang, Shi Lei, Fang Yujiang, Ma Lin, Liu Yang, Terheyden-Keighley Daniel, Liu Ling, Zhang Xiaoqing

Abstract excerpt

It is highly desirable to specify human developmental principles in an appropriate human model with advanced genetic tools. However, genetically engineering human cells with lineage-tracing systems has not been achieved. Here we introduce strategies to construct lineage-tracing systems in human embryonic stem cells (hESCs). The AAVS1 locus was suitable for the integration of the conditional reporter. The Cre-LoxP...

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Identifiers

PMID: 30449321
DOI: 10.1016/j.stemcr.2018.09.014
PMCID: PMC6234918

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PAX6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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