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Article

Analysis of red-fluorescent proteins provides insight into dark-state conversion and photodegradation.

Biophysical journal - 17 Aug 2011

Dean Kevin M, Lubbeck Jennifer L, Binder Jennifer K, Schwall Linda R, Jimenez Ralph, Palmer Amy E

Abstract excerpt

Fluorescent proteins (FPs) are powerful tools that permit real-time visualization of cellular processes. The utility of a given FP for a specific experiment depends strongly on its effective brightness and overall photostability. However, the brightness of FPs is limited by dark-state conversion (DSC) and irreversible photobleaching, which occur on different timescales. Here, we present in vivo ensemble assays...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 21843488
DOI: 10.1016/j.bpj.2011.06.055
PMCID: PMC3175071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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