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Development and characterization of green fluorescent protein mutants with altered lifetimes.

Biochemistry - 11 Oct 2005

Scruggs Allan W, Flores Carole L, Wachter Rebekka, Woodbury Neal W

Abstract excerpt

Multiple-probe fluorescence imaging applications demand an ever-increasing number of resolvable probes, and the use of fluorophores with resolvable fluorescence lifetimes can help meet this demand. Green fluorescent protein (GFP) and its variants have been widely used in spectrally resolved multiprobe imaging, but as yet, there has not been a systematic set of mutants generated with resolvable lifetimes....

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Biochemistry
Cysteine
Genetic Techniques
Histidine
Microscopy, Fluorescence

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Models, Chemical

Spectrophotometry

Ultraviolet Rays

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PMID: 16201762
DOI: 10.1021/bi050550f

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