Article
Tuning the specificity of DNA probes using bulge-loops for low-abundance SNV detection.
Biosensors & bioelectronics - 15 Apr 2020
Bai Shulian, Xu Bangtian, Zhang Yangli, Zhang Yuhong, Dang Hao, Yang Shuangshuang, Zuo Chen, Zhang Li, Li Junjie, Xie Guoming
Abstract excerpt
Tuning the free energy difference between a molecular probe and the target has been regarded as a feasible way to realize selective mutant recognition. But due to limited extent of variation on the probing sequences, it remains a challenge to moderately leverage the thermodynamic kinetics simply by changing the base composition of probes. Herein we propose the modulation of discrimination capability for single...
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