Article
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform.
Nature communications - 26 Jun 2014
Yoshimi K, Kaneko T, Voigt B, Mashimo T
Abstract excerpt
The bacterial CRISPR/Cas system has proven to be an efficient gene-targeting tool in various organisms. Here we employ CRISPR/Cas for accurate and efficient genome editing in rats. The synthetic chimeric guide RNAs (gRNAs) discriminate a single-nucleotide polymorphism (SNP) difference in rat embryonic fibroblasts, allowing allele-specific genome editing of the dominant phenotype in (F344 × DA)F1 hybrid embryos....
Topics
Join the communities discussing this publication.