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Article

High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics.

Genome research - 1 Sept 2018

Pellegrino Maurizio, Sciambi Adam, Treusch Sebastian, Durruthy-Durruthy Robert, Gokhale Kaustubh, Jacob Jose, Chen Tina X, Geis Jennifer A, Oldham William, Matthews Jairo, Kantarjian Hagop, Futreal P Andrew, Patel Keyur, Jones Keith W, Takahashi Koichi, Eastburn Dennis J

Abstract excerpt

To enable the characterization of genetic heterogeneity in tumor cell populations, we developed a novel microfluidic approach that barcodes amplified genomic DNA from thousands of individual cancer cells confined to droplets. The barcodes are then used to reassemble the genetic profiles of cells from next-generation sequencing data. By using this approach, we sequenced longitudinally collected acute myeloid...

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Identifiers

PMID: 30087104
DOI: 10.1101/gr.232272.117
PMCID: PMC6120635

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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