Article
Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level.
Cancer science - 1 May 2021
Nakamura Ikuko Takeda, Ikegami Masachika, Hasegawa Nobuhiko, Hayashi Takuo, Ueno Toshihide, Kawazu Masahito, Yagishita Shigehiro, Goto Yasushi, Shinno Yuki, Kojima Yuki, Takamochi Kazuya, Takahashi Fumiyuki, Takahashi Kazuhisa, Mano Hiroyuki, Kohsaka Shinji
Abstract excerpt
Liquid biopsy analyzes the current status of primary tumors and their metastatic regions. We aimed to develop an optimized protocol for single-cell sequencing of floating tumor cells (FTCs) in pleural effusion as a laboratory test. FTCs were enriched using a negative selection of white blood cells by a magnetic-activated cell sorting system, and CD45-negative and cytokeratin-positive selection using a...
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