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Article

Rational engineering of Cel5E from Clostridium thermocellum to improve its thermal stability and catalytic activity.

Applied microbiology and biotechnology - 1 Oct 2018

Torktaz Ibrahim, Karkhane Ali Asghar, Hemmat Jafar

Abstract excerpt

The celH gene from Clostridium thermocellum encodes a protein containing 900 residues and three components, including Cel5E, Lic26a, and carbohydrate-binding domains. Cel5E is a member of the glycoside hydrolase-5 family and is a bifunctional xylanase/cellulase enzyme. We targeted a semi-hydrophobic pocket near the Cel5E active site and theoretically screened mutated variants for enhanced levels of thermal...

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PMID: 30074072
DOI: 10.1007/s00253-018-9204-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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