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Identification and Mutation Analysis of Nonconserved Residues on the TIM-Barrel Surface of GH5_5 Cellulases for Catalytic Efficiency and Stability Improvement.

Applied and environmental microbiology - 13 Sept 2022

Zheng Jie, Liu Han-Qing, Qin Xing, Yang Kun, Tian Jian, Wang Xiao-Lu, Wang Ya-Ru, Wang Yuan, Yao Bin, Luo Hui-Ying, Huang Huo-Qing

Abstract excerpt

Exploring the potential functions of nonconserved residues on the outer side of α-helices and systematically optimizing them are pivotal for their application in protein engineering. Based on the evolutionary structural conservation analysis of GH5_5 cellulases, a practical molecular improvement strategy was developed. Highly variable sites on the outer side of the α-helices of the GH5_5 cellulase from...

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PMID: 36000858
DOI: 10.1128/aem.01046-22
PMCID: PMC9469711

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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