Rational Engineering of a β-Glucosidase (H0HC94) from Glycosyl Hydrolase Family I (GH1) to Improve Catalytic Performance on Cellobiose.

doi:10.1021/acs.jpcb.4c03464

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Article

Rational Engineering of a β-Glucosidase (H0HC94) from Glycosyl Hydrolase Family I (GH1) to Improve Catalytic Performance on Cellobiose.

The journal of physical chemistry. B - 12 Sept 2024

Sengupta Sauratej, Chanda Pinaki, Manna Bharat, Ghosh Amit, Datta Supratim

Abstract excerpt

The conversion of lignocellulosic feedstocks by cellulases to glucose is a critical step in biofuel production. β-Glucosidases catalyze the final step in cellulose breakdown, producing glucose, and are often the rate-limiting step in biomass hydrolysis. The specific activity of most natural and engineered β-glucosidase is higher on the artificial substrate p-nitrophenyl β-d-glucopyranoside (pNPGlc) than on the...

Topics

Protein Engineering
Biocatalysis
Cellobiose
Models, Molecular
Molecular Dynamics Simulation
beta-Glucosidase
Substrate Specificity
Mutation
Enzyme Stability
Temperature
Bacteria
Hydrogen Bonding

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Open in PubMed

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PMID: 39221646
DOI: 10.1021/acs.jpcb.4c03464

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