Article
Structural Insight of a Trimodular Halophilic Cellulase with a Family 46 Carbohydrate-Binding Module.
PloS one - 1 Jan 2015
Zhang Huaidong, Zhang Guimin, Yao Chaoxiang, Junaid Muhammad, Lu Zhenghui, Zhang Houjin, Ma Yanhe
Abstract excerpt
Cellulases are the key enzymes used in the biofuel industry. A typical cellulase contains a catalytic domain connected to a carbohydrate-binding module (CBM) through a flexible linker. Here we report the structure of an atypical trimodular cellulase which harbors a catalytic domain, a CBM46 domain and a rigid CBM_X domain between them. The catalytic domain shows the features of GH5 family, while the CBM46 domain...
Topics
- Amino Acid Sequence
- Bacillus
- Bacterial Proteins
- Binding Sites
- Carbohydrates
- Catalytic Domain
- Cellulase
- Crystallography, X-Ray
- Molecular Dynamics Simulation
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Structure, Tertiary