Article
Mechanistic insights into activity-enhancing mutations from comparative analysis of two Bacillus subtilis GH11 xylanases.
International journal of biological macromolecules - 1 Apr 2026
Liu Changjun, He Shaolong, Qu Cong, Zhang Zheyuan, Duan Xinyi, Yu Zhouyiling, Zhang Xingyi, Lu Yuyao, Zhang Dongyi, Meng Er
Abstract excerpt
GH11 Xylanases are valuable biocatalysts for xylooligosaccharides production. Enhancing catalytic activity is one of the primary goals in xylanase engineering. Leveraging evolutionary information through comparative analysis of homologous sequences offers a promising strategy to identify key residues for modification, thereby improving enzyme performance and providing insights for rational design. In this study,...
Topics
- Bacillus subtilis
- Endo-1,4-beta Xylanases
- Molecular Dynamics Simulation
- Enzyme Stability
- Mutation
- Kinetics
- Substrate Specificity
- Hydrogen-Ion Concentration
- Amino Acid Sequence