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Article

Engineering the affinity of a family 11 carbohydrate binding module to improve binding of branched over unbranched polysaccharides.

International journal of biological macromolecules - 1 Dec 2018

Furtado Gilvan Pessoa, Lourenzoni Marcos Roberto, Fuzo Carlos Alessandro, Fonseca-Maldonado Raquel, Guazzaroni María-Eugenia, Ribeiro Lucas Ferreira, Ward Richard J

Abstract excerpt

Carbohydrate binding modules (CBMs) are non-catalytic domains within larger multidomain polypeptides. The CelH from Ruminoclostridium (Clostridium) thermocellum contains a family 11 CBM (RtCBM11) with high binding affinity for the linear polysaccharide β-glucan, and low affinity for the branched xyloglucan. Screening a random RtCBM11 mutant phage library created by error prone PCR for xyloglucan binding...

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PMID: 30195003
DOI: 10.1016/j.ijbiomac.2018.09.022

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