Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

[Expression and purification of FLT3 and FLT3-ITD mutated proteins in HEK293T cells].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Mar 2018

Yang Fan, Du Jiahui, Wu Jie, Yuan Puyu, Su Qian, Han Rong, Qiu Qiaocheng, Xue Shengli, Liu Songbai, Sun Zhongwen

Abstract excerpt

Objective To construct the eukaryotic expression vectors of human fms related tyrosine kinase 3 (FLT3) gene and FLT3-internal tandem duplication (FLT3-ITD) mutants and purify the native proteins through immunoprecipitation from HEK293T cell lysates. Methods The cDNA fragments of FLT3wt and FLT3-ITD were amplified from bone marrow cells of healthy individuals and FLT3-ITD-mutated acute myeloid leukemia (AML)...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mutation
Share to Blotting, Western

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Bone Marrow Cells

Share to fms-Like Tyrosine Kinase 3

Share to Gene Expression

Share to FLT3 gene

Share to HEK293 Cells

Share to Leukemia, Myeloid, Acute

Source

Identifiers

PMID: 29773106

Genes

FLT3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

[Expression and purification of FLT3 and FLT3-ITD mutated proteins in HEK293T cells].PMID 29773106Read ↗

Expression of human vascular endothelial growth factor receptor Flt-1 extracellular domain 1-3 loop cDNA in Pichia pastoris, purification of the expressed product and detection of its biological activity.Semantic proximity Read ↗

Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs.Semantic proximity Read ↗

Identification of Flt3 internal tandem duplications downstream targets by high-throughput immunoblotting protein array system.Semantic proximity Read ↗

Lipopolymer/siRNA complexes engineered for optimal molecular and functional response with chemotherapy in FLT3-mutated acute myeloid leukemia.Semantic proximity Read ↗

Importance of PTM of FLT3 in acute myeloid leukemia.Semantic proximity Read ↗

Generation and characterization of a highly effective protein substrate for analysis of FLT3 activity.Semantic proximity Read ↗

Wild-type FLT3 and FLT3 ITD exhibit similar ligand-induced internalization characteristics.Semantic proximity Read ↗

Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cellsSemantic proximity Read ↗

[Effect of FLT3-ITD Length on 32D Cell Proliferation, Apoptosis and Sensitivity to FLT3 Inhibitor].Semantic proximity Read ↗

[Construction of expression vector pLCK-CD69-IRES-EGFP and generation of CD69 transgenic mice].Semantic proximity Read ↗

Biochemical and genetic characterization of multiple splice variants of the Flt3 ligandSemantic proximity Read ↗

Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.