Article
Expression of human vascular endothelial growth factor receptor Flt-1 extracellular domain 1-3 loop cDNA in Pichia pastoris, purification of the expressed product and detection of its biological activity.
Biomedical and environmental sciences : BES - 1 Dec 2001
Ma L, Wang X N, Zhang Z Q, Zhou X M, Zeng G F, Chen A J
Abstract excerpt
OBJECTIVE: To express human vascular endothelial growth factor receptor Flt-1 extracellular domain 1-3 loop cDNA in Pichia. pastroris, and to purify the expressed product and detect its biological activity. METHODS: By inserting human Flt-1 (1-3 loop) cDNA coding 316 amino acid residues into Pichia pastoris expression vector pPIC9K containing AOX1 promoter and the sequences of alpha secreting signal peptides, a...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.