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Article

Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system.

PloS one - 1 Jan 2022

Yamamoto Naoki, Kikuchi Jiro, Furukawa Yusuke, Shibayama Naoya

Abstract excerpt

We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay...

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Identifiers

PMID: 35511790
DOI: 10.1371/journal.pone.0261699
PMCID: PMC9070948

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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