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Article

Mutation screening using formalin-fixed paraffin-embedded tissues: a stratified approach according to DNA quality.

Laboratory investigation; a journal of technical methods and pathology - 1 Aug 2018

Cucco Francesco, Clipson Alexandra, Kennedy Hannah, Sneath Thompson Joe, Wang Ming, Barrans Sharon, van Hoppe Moniek, Ochoa Ruiz Eguzkine, Caddy Josh, Hamid Debbie, Cummin Thomas, Burton Cathy, Davies Andrew J, Johnson Peter, Du Ming-Qing

Abstract excerpt

DNA samples from formalin-fixed paraffin-embedded tissues are highly degraded with variable quality, and this imposes a big challenge for targeted sequencing due to false positives, largely caused by PCR errors and cytosine deamination. To eliminate false positives, a common practice is to validate the detected variants by Sanger sequencing or perform targeted sequencing in duplicate. Technically, PCR errors...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29769698
DOI: 10.1038/s41374-018-0066-z

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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