Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evaluation of two highly-multiplexed custom panels for massively parallel semiconductor sequencing on paraffin DNA.

PloS one - 1 Jan 2015

Kotoula Vassiliki, Lyberopoulou Aggeliki, Papadopoulou Kyriaki, Charalambous Elpida, Alexopoulou Zoi, Gakou Chryssa, Lakis Sotiris, Tsolaki Eleftheria, Lilakos Konstantinos, Fountzilas George

Abstract excerpt

BACKGROUND: AIM: Massively parallel sequencing (MPS) holds promise for expanding cancer translational research and diagnostics. As yet, it has been applied on paraffin DNA (FFPE) with commercially available highly multiplexed gene panels (100s of DNA targets), while custom panels of low multiplexing are used for re-sequencing. Here, we evaluated the performance of two highly multiplexed custom panels on FFPE...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Breast Neoplasms
Share to DNA, Neoplasm

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Formaldehyde

Share to High-Throughput Nucleotide Sequencing

Share to Neoplasm Proteins

Share to Paraffin Embedding

Share to Pilot Projects

Share to Tissue Fixation

Source

Open in PubMed Open full text

Identifiers

PMID: 26039550
DOI: 10.1371/journal.pone.0128818
PMCID: PMC4454570

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evaluation of two highly-multiplexed custom panels for massively parallel semiconductor sequencing on paraffin DNA.PMID 26039550Read ↗

Massive parallel amplicon sequencing of the breast cancer genes BRCA1 and BRCA2: opportunities, challenges, and limitations.Semantic proximity Read ↗

Towards a Next-Generation Sequencing Diagnostic Service for Tumour Genotyping: A Comparison of Panels and Platforms.Semantic proximity Read ↗

Benchmarking whole exome sequencing in the German network for personalized medicineSemantic proximity Read ↗

Use of FFPE-Derived DNA in Next Generation Sequencing: DNA extraction methodsSemantic proximity Read ↗

Massive parallel sequencing of solid tumours – challenges and opportunities for pathologistsSemantic proximity Read ↗

Multicenter In-House Evaluation of an Amplicon-Based Next-Generation Sequencing Panel for Comprehensive Molecular Profiling.Semantic proximity Read ↗

Use of FFPE-derived DNA in next generation sequencing: DNA extraction methodsSemantic proximity Read ↗

Genetic testing in hereditary breast and ovarian cancer using massive parallel sequencing.Semantic proximity Read ↗

Implementation of Amplicon Parallel Sequencing Leads to Improvement of Diagnosis and Therapy of Lung Cancer PatientsSemantic proximity Read ↗

Cross-laboratory validation of the OncoScan® FFPE Assay, a multiplex tool for whole genome tumour profiling.Semantic proximity Read ↗

Targeted Next Generation Sequencing as a Reliable Diagnostic Assay for the Detection of Somatic Mutations in Tumours Using Minimal DNA Amounts from Formalin Fixed Paraffin Embedded Material.Semantic proximity Read ↗

Development and Validation of StrataNGS, a Multiplex PCR, Semiconductor Sequencing-Based Comprehensive Genomic Profiling TestSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.