Article
Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization.
Protein engineering, design & selection : PEDS - 1 Jul 2018
Schwaigerlehner L, Pechlaner M, Mayrhofer P, Oostenbrink C, Kunert R
Abstract excerpt
Humanized monoclonal antibodies (mAbs) are among the most promising modern therapeutics, but defined engineering strategies are still not available. Antibody humanization often leads to a loss of affinity, as it is the case for our model antibody Ab2/3H6 (PDB entry 3BQU). Identifying appropriate back-to-mouse mutations is needed to restore binding affinity, but highly challenging. In order to get more insight, we...
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