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Increasing the melting temperature of VHH with the in silico free energy score.

Scientific reports - 25 Mar 2023

Tomimoto Yusuke, Yamazaki Rika, Shirai Hiroki

Abstract excerpt

VHH, the antigen-binding fragment of a heavy chain-only antibody, is a useful component of antibody-based therapeutics. Thermal stability, represented by the melting temperature (Tm), is one of the key factors affecting the developability of antibody-based therapeutics. In this study, we examined whether the in silico free energy score dStability can be used to design mutants with improved Tm compared to the...

Topics

Temperature
Entropy
Antibodies
Mutation
Mutagenesis

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 36966210
DOI: 10.1038/s41598-023-32022-8
PMCID: PMC10039853

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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