Article
Affinity maturation of antibody fragments: A review encompassing the development from random approaches to computational rational optimization.
International journal of biological macromolecules - 30 Aug 2023
Li Jiaqi, Kang Guangbo, Wang Jiewen, Yuan Haibin, Wu Yili, Meng Shuxian, Wang Ping, Zhang Miao, Wang Yuli, Feng Yuanhang, Huang He, de Marco Ario
Abstract excerpt
Routinely screened antibody fragments usually require further in vitro maturation to achieve the desired biophysical properties. Blind in vitro strategies can produce improved ligands by introducing random mutations into the original sequences and selecting the resulting clones under more and more stringent conditions. Rational approaches exploit an alternative perspective that aims first at identifying the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.