Article
Detection of genome-edited cells by oligoribonucleotide interference-PCR.
DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Aug 2018
Fujita Toshitsugu, Yuno Miyuki, Kitaura Fusako, Fujii Hodaka
Abstract excerpt
Genome editing by engineered sequence-specific nucleases, such as the clustered regularly interspaced short palindromic repeats (CRISPR) system is widely used for analysis of gene functions. Several techniques have been developed for detection of genome-edited cells, but simple, cost-effective, and positive detection methods remain limited. Recently, we developed oligoribonucleotide (ORN) interference-PCR...
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