Article
Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes.
Plant biotechnology journal - 1 Dec 2018
Liang Zhen, Chen Kunling, Yan Yan, Zhang Yi, Gao Caixia
Abstract excerpt
Despite the great achievements in genome editing, accurately detecting mutations induced by sequence-specific nucleases is still a challenge in plants, especially in polyploidy plants. An efficient detection method is particularly vital when the mutation frequency is low or when a large population needs to be screened. Here, we applied purified CRISPR ribonucleoprotein complexes to cleave PCR products for...
Topics
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- DNA, Plant
- Gene Editing
- Genotyping Techniques
- Mutation
- Polymerase Chain Reaction
- Ribonucleoproteins
- Sequence Analysis, DNA
- Triticum