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Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR

14 Dec 2020

Abstract excerpt

Gene-editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been reported that detect mutations at targeted loci induced by the CRISPR/Cas system in different organisms, they are semiquantitative and have difficulty in the detection of mutants in processed food samples containing low initial concentrations of DNA and may not accurately quantify...

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PMID: 33381141
DOI: 10.3389/fpls.2020.610790

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