Article
Extreme assay sensitivity in molecular diagnostics further unveils intratumour heterogeneity in metastatic colorectal cancer as well as artifactual low-frequency mutations in the KRAS gene.
British journal of cancer - 25 Jul 2017
Mariani Sara, Bertero Luca, Osella-Abate Simona, Di Bello Cristiana, Francia di Celle Paola, Coppola Vittoria, Sapino Anna, Cassoni Paola, Marchiò Caterina
Abstract excerpt
BACKGROUND: Gene mutations in the RAS family rule out metastatic colorectal carcinomas (mCRCs) from anti-EGFR therapies. METHODS: We report a retrospective analysis by Sequenom Massarray and fast COLD-PCR followed by Sanger sequencing on 240 mCRCs. RESULTS: By Sequenom, KRAS and NRAS exons 2-3-4 were mutated in 52.9% (127/240) of tumours, while BRAF codon 600 mutations reached 5% (12/240). Fast COLD-PCR found...
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