Article
Systematic analysis of complex genetic interactions.
Science (New York, N.Y.) - 20 Apr 2018
Kuzmin Elena, VanderSluis Benjamin, Wang Wen, Tan Guihong, Deshpande Raamesh, Chen Yiqun, Usaj Matej, Balint Attila, Mattiazzi Usaj Mojca, van Leeuwen Jolanda, Koch Elizabeth N, Pons Carles, Dagilis Andrius J, Pryszlak Michael, Wang Zi Yang, Hanchard Julia, Riggi Margot, Xu Kaicong, Heydari Hamed, San Luis Bryan-Joseph, Shuteriqi Ermira, Zhu Hongwei, Van Dyk Nydia, Sharifpoor Sara, Costanzo Michael, Loewith Robbie, Caudy Amy, Bolnick Daniel, Brown Grant W, Andrews Brenda J, Boone Charles, Myers Chad L
Abstract excerpt
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred...
Topics
- Gene Regulatory Networks
- Mutation
- Oligonucleotide Array Sequence Analysis
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins