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Article

Bifunctional role of leucine 300 of firefly luciferase in structural rigidity.

International journal of biological macromolecules - 1 Aug 2017

Yousefi Farzad, Ataei Farangis, Mortazavi Mojtaba, Hosseinkhani Saman

Abstract excerpt

Firefly luciferase is susceptible to thermal inactivation, thereby its intracellular half-life decreased. Previous reports indicated that L300R mutation (LRR mutant) in E354R/Arg356 double mutant (ERR mutant) from Lampyris turkestanicus luciferase has increased its thermal stability and rigidity through induction of some ionic bonds with Asp 270 and 271. Disruption of the deduced ionic bonds in an ultra-rigid...

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Identifiers

PMID: 28322945
DOI: 10.1016/j.ijbiomac.2017.03.069

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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