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Article

In vivo incorporation of an alkyne into proteins in Escherichia coli.

Bioorganic & medicinal chemistry letters - 1 Mar 2005

Deiters Alexander, Schultz Peter G

Abstract excerpt

Using a genetic selection we identified mutants of the M. janaschii tyrosyl-tRNA synthetase that selectively charge an amber suppressor tRNA with para-propargyloxyphenylalanine in Escherichia coli. These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild...

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Identifiers

PMID: 15713420
DOI: 10.1016/j.bmcl.2004.12.065

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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