Article
The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.
International journal of molecular sciences - 4 Apr 2018
Watanabe Satoshi, Sakurai Takayuki, Nakamura Shingo, Miyoshi Kazuchika, Sato Masahiro
Abstract excerpt
Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) have facilitated genomic modification in mammalian cells. However, most systems employ transient treatment with selective drugs such as puromycin to obtain the desired genome-edited cells, which often allows some untransfected cells to survive and decreases...
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