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Article

Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system.

Methods (San Diego, Calif.) - 1 Sept 2014

Zhang Yu, Vanoli Fabio, LaRocque Jeannine R, Krawczyk Przemek M, Jasin Maria

Abstract excerpt

Gene targeting - homologous recombination between transfected DNA and a chromosomal locus - is greatly stimulated by a DNA break in the target locus. Recently, the RNA-guided Cas9 endonuclease, involved in bacterial adaptive immunity, has been modified to function in mammalian cells. Unlike other...

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Identifiers

PMID: 24929070
DOI: 10.1016/j.ymeth.2014.05.003
PMCID: PMC4405113

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-24929070-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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