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Article

Concurrent In Vitro Synthesis and Functional Detection of Nascent Activity of the KcsA Channel under a Membrane Potential.

ACS synthetic biology - 20 Apr 2018

Iwamoto Masayuki, Elfaramawy Maie A, Yamatake Mariko, Matsuura Tomoaki, Oiki Shigetoshi

Abstract excerpt

Processes involved in the functional formation of prokaryotic membrane proteins have remained elusive. Here, we developed a new in vitro membrane protein expression system to detect nascent activities of the KcsA potassium channel in lipid bilayers under an applied membrane potential. The channel was synthesized using a reconstituted Escherichia coli-based in vitro transcription/translation system (IVTT) in a...

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Identifiers

PMID: 29566487
DOI: 10.1021/acssynbio.7b00454

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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