Article
In vitro synthesis and oligomerization of the mechanosensitive channel of large conductance, MscL, into a functional ion channel.
FEBS letters - 3 Jan 2011
Price Claire E, Kocer Armagan, Kol Stefan, van der Berg Jan Pieter, Driessen Arnold J M
Abstract excerpt
Elucidation of high-resolution structures of integral membrane proteins is drastically lagging behind that of cytoplasmic proteins. In vitro synthesis and insertion of membrane proteins into synthetic membranes could circumvent bottlenecks associated with the overexpression of membrane proteins, producing sufficient membrane-inserted, correctly folded protein for structural studies. Using the mechanosensitive...
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