Article
Estimation of pyrazinamidase activity using a cell-free In vitro synthesis of pnca and its association with pyrazinamide susceptibility in Mycobacterium tuberculosis.
International journal of mycobacteriology - 1 Jan 2000
Rueda Daniel, Bernard Christine, Gandy Lucas, Capton Estelle, Boudjelloul Rachid, Brossier Florence, Veziris Nicolas, Zimic Mirko, Sougakoff Wladimir
Abstract excerpt
Background: The main mechanism of resistance to PZA in Mycobacterium tuberculosis relies on mutations on its pyrazinamidase/nicotinamidase. Recently, a rapid colorimetric test relying on the PCR-based in vitro-synthesized-PZase assay has been reported for PZase activity determination from clinical M. tuberculosis isolates but the assay has not been compared with other tests to evaluate PZA susceptibility in M....
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